Enzymes and Inhibitors
T4 RNA ligase II, 750 U, G-Biosciences
T4 RNA Ligase II (gp24.1) repairs nicks in dsRNA and can also be used to ligate the 3' terminal OH of RNA to the 5'-terminal phosphate of DNA in a double-stranded structure.
E. coli Pyrophosphatase (PPase), 50 U, G-Biosciences
E. coli Inorganic Pyrophosphatase (PPase) speeds up the breakdown of inorganic pyrophosphate (PPi) into orthophosphate.
Alkaline Phosphatase, 2500 U, G-Biosciences
TAB5 Alkaline Phosphatase is a heat labile alkaline phosphatase. It catalyzes the dephosphorylation of phosphomonoesters at both 5' and 3' ends of DNA and RNA molecules in a nonspecific manner. Additionally, it eliminates phosphate groups from the 5' and 3' positions of DNA, RNA, and dNTPs.
Klenow Exo, 1000 U, G-Biosciences
Klenow fragment (Exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′→5′) and nick-translation (5′→3′) nuclease activities, with a moderate strand displacement activity during DNA synthesis.
G-Biosciences T5 Exonuclease
T5 Exonuclease is a highly efficient 5'-to-3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions.
Endonuclease VIII, 2000 U, G-Biosciences
Endonuclease VIII from E. coli has dual functions as an N-glycosylase and an AP-lyase. It removes damaged pyrimidines from double-stranded DNA to create an AP site. The AP-lyase activity then cleaves at this site, resulting in a 5′ phosphate and a 3′ phosphate.
E. coli Exonuclease I, 5000 U, G-Biosciences
Exonuclease I (Exo I), is a highly processive single-stranded DNA-specific exonuclease which removes mono- and dinucleotides from the 3' end of single-stranded DNA molecules (3' to 5' exonuclease activity) leaving double-stranded molecules and the 5' end of single stranded molecules intact.
Thermolabile UDG, 500 U, G-Biosciences
Thermolabile Uracil-DNA Glycosylase (UDG), originating from the species Gadus morhua, removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site.
RNase R, 500 U, G-Biosciences
A C-terminal truncated version of the E.coli RNase R. This truncated enzyme exhibits higher specific activity compared to the full length enzyme.
G-Biosciences RNase Inhibitor (Murine)
RNase Inhibitor (Murine) is used for first strand cDNA synthesis, RT-qPCR.
Cytiva Stainless steel pressure vessel, 18.9 L, 5 Gallon Capacity, 1/pk
Stainless steel pressure vessel, 18.9 L, 5 Gallon Capacity, 1/pk
New England Biolabs, Inc. SpeI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
BIOSEARCH TECHNOLOGIES INC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 1000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.